Vol. 2 No. 1 (2026): April
Open Access
Peer Reviewed

Comparison of Antibacterial Activity and Physicochemical Characteristics of Vco (Virgin Coconut Oil) With Different Manufacturing Methods

Authors

Hana Safitri Putri Hadi , Sarkono , A.A. Ngurah Nara Kusuma , Muh. Azhar Kholidi

DOI:

10.65622/ijtb.v2i1.279

Downloads

Received: 2026-05-04
Accepted: 2026-05-15
Published: 2026-05-15

Abstract

Cocos nucifera is an important plantation commodity in Indonesia with high economic value and considerable potential for the development of value-added products, including Virgin Coconut Oil. This study aimed to compare the physicochemical characteristics and antibacterial activity of VCO produced using heating, fermentation, and enzymatic methods. The research was conducted experimentally from June to August 2024 at the Advanced Biology Laboratory, Faculty of Mathematics and Natural Sciences, University of Mataram. VCO samples were evaluated through organoleptic tests and physicochemical analyses, including yield, moisture content, peroxide value, and free fatty acid content. Antibacterial activity was tested against Staphylococcus aureus and Escherichia coli using the disk diffusion method. The results showed that all VCO samples generally met Indonesian National Standard quality requirements, except for free fatty acid content in some treatments. The fermentation method produced the highest yield (31.88%), while the heating method resulted in the best overall quality, characterized by 0% moisture content, 0 mg Eq/kg peroxide value, and the highest organoleptic acceptance. All VCO samples exhibited antibacterial activity, although the inhibition zones were categorized as weak. The findings indicate that the heating method is the most suitable for producing high-quality VCO with antibacterial potential.

Keywords:

Antibacterial activity Physicochemical properties Virgin Coconut Oil

References

Agus, H. N., Pravitasari, A. E., & Supijatno. (2025). Development strategy for smallholder oil palm and coconut plantation in Pesisir Selatan Regency. Journal of the Saudi Society of Agricultural Sciences, 24(5), 35. https://doi.org/10.1007/s44447-025-00033-8

Ailli, A., Zibouh, K., Eddamsyry, B., Drioiche, A., Fetjah, D., Ayyad, F. Z., Mothana, R. A., Hawwal, M. F., Radi, M., Tarik, R., Elomri, A., Mouradi, A., & Zair, T. (2024). Physicochemical Characterization of Moroccan Honey Varieties from the Fez-Meknes Region and Their Antioxidant and Antibacterial Properties. Metabolites, 14(7), 364. https://doi.org/10.3390/metabo14070364

Bazina, N., Ahmed, T., Almdaaf, M., Abu Hallalah, H. M. O., & Jibia, S. (2025). Chemical Changes in Deep‐Fat Frying: Reaction Mechanisms, Oil Degradation, and Health Implications. Food Science & Nutrition, 13(10). https://doi.org/10.1002/fsn3.70969

David, S. R., & Rajabalaya, R. (2024). Development and in vitro Evaluation of Nanoemulsion-based Polyherbal Hair Serum Formulations for Leave-in Hair Treatments. Current Cosmetic Science, 03 https://doi.org/10.2174/012666779

Divate, A. D., Moses, J. A., Anandakumar, S., Sinija, V. R., & Venkatachalapathy, N. (2026). Toward sustainability in coconut processing: current challenges, emerging concepts, and circular solutions. Sustainable Food Technology. https://doi.org/10.1039/D5FB00508F

Djalil, A. D., Setyawan, H., Gumelar, M. I., Nurulita, N. A., & Budiman, A. (2019). Antioxidant potentials of virgin olive oil and virgin coconut oil and its cream formulation. Journal of Physics: Conference Series, 1402(5), 055067. https://doi.org/10.1088/1742-6596/1402/5/055067

Duman, H., Eker, F., Akdaşçi, E., Witkowska, A. M., Bechelany, M., & Karav, S. (2024). Silver Nanoparticles: A Comprehensive Review of Synthesis Methods and Chemical and Physical Properties. Nanomaterials, 14(18), 1527. https://doi.org/10.3390/nano14181527

Gupta, V., Vijayalakshmi, N. S., Ashwini, B., Anbarasu, K., Vijayalakshmi, G., Prakash, M., Indiramma, A. R., Rangarao, G. C. P., & Ramesh, B. S. (2010). Shelf Life Enhancement Of Coconut Burfi – An Indian Traditional Sweet. Journal of Food Quality, 33(3), 329–349. https://doi.org/10.1111/j.1745-4557.2010.00312.x

Habiba, U., Bajpai, A., Shafi, Z., Pandey, V. K., & Singh, R. (2025). Advancing sustainability through modified atmosphere packaging (MAP) for fresh food preservation: A critical review. Journal of Stored Products Research, 112, 102657. https://doi.org/10.1016/j.jspr.2025.102657

He, Q., Peng, H., Sheng, M., Hu, S., Qiu, J., & Gu, J. (2019). Humidity Control Strategies for Solid-State Fermentation: Capillary Water Supply by Water-Retention Materials and Negative-Pressure Auto-controlled Irrigation. Frontiers in Bioengineering and Biotechnology, 7. https://doi.org/10.3389/fbioe.2019.00263

Jakfar, Husin, H., Pontas, K., Mamat, R., Salleh, M. R., Zulrika, M., & Ahmadi. (2023). Modification of the Fermentation Process and Papain Enzymes in The Manufacture of Virgin Coconut Oil Using Optimization of Response Surface Methodology, Central Composite Design. Fermentation, 9(5), 434. https://doi.org/10.3390/fermentation9050434

Klančnik, A., Piskernik, S., Jeršek, B., & Možina, S. S. (2010). Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. Journal of Microbiological Methods, 81(2), 121–126. https://doi.org/10.1016/j.mimet.2010.02.004

Kozłowska, M., Ziarno, M., Rudzińska, M., Majcher, M., Małajowicz, J., & Michewicz, K. (2022). The Effect of Essential Oils on the Survival of Bifidobacterium in In Vitro Conditions and in Fermented Cream. Applied Sciences, 12(3), 1067. https://doi.org/10.3390/app12031067

Makimoto, Y., Nabeshima, K., Iwasaki, H., Miyoshi, T., Enatsu, S., Shiraishi, T., Iwasaki, A., Shirakusa, T., & Kikuchi, M. (2005). Micropapillary pattern: a distinct pathological marker to subclassify tumours with a significantly poor prognosis within small peripheral lung adenocarcinoma (≤20 mm) with mixed bronchioloalveolar and invasive subtypes (Noguchi’s type C tumours). Histopathology, 46(6), 677–684. https://doi.org/10.1111/j.1365-2559.2005.02126.x

Mohammed, N. K., Samir, Z. T., Jassim, M. A., & Saeed, S. K. (2021). Effect of different extraction methods on physicochemical properties, antioxidant activity, of virgin coconut oil. Materials Today: Proceedings, 42, 2000–2005. https://doi.org/10.1016/j.matpr.2020.12.248

Mutz, D. C., & Pemantle, R. (2015). Standards for Experimental Research: Encouraging a Better Understanding of Experimental Methods. Journal of Experimental Political Science, 2(2), 192–215. https://doi.org/10.1017/XPS.2015.4.

Naraharasetti, B., Chakraborty, S., Siliveru, K., & Prasad, P. V. V. (2025). Thermal and Nonthermal Processing of Pearl Millet Flour: Impact on Microbial Safety, Enzymatic Stability, Nutrients, Functional Properties, and Shelf‐Life Extension. Comprehensive Reviews in Food Science and Food Safety, 24(3). https://doi.org/10.1111/1541-4337.70190

Nasir, N. A. M. M., Abllah, Z., Azura Jalaludin, A., Azura Shahdan, I., & Hayati Wan Abd Manan, W. N. (2018). Virgin Coconut Oil and Its Antimicrobial Properties against Pathogenic Microorganisms: A Review. Proceedings of the International Dental Conference of Sumatera Utara 2017 (IDCSU 2017). https://doi.org/10.2991/idcsu-17.2018.51

Nasution, Z., Jirapakkul, W., & Lorjaroenphon, Y. (2019). Aroma compound profile of mature coconut water from tall variety through thermal treatment. Journal of Food Measurement and Characterization, 13(1), 277–286. https://doi.org/10.1007/s11694-018-9942-x

Neagu, R., Popovici, V., Ionescu, L.-E., Ordeanu, V., Biță, A., Popescu, D. M., Ozon, E. A., & Gîrd, C. E. (2024). Phytochemical Screening and Antibacterial Activity of Commercially Available Essential Oils Combinations with Conventional Antibiotics against Gram-Positive and Gram-Negative Bacteria. Antibiotics, 13(6), 478. https://doi.org/10.3390/antibiotics13060478

Nitbani, F. O., Tjitda, P. J. P., Nitti, F., Jumina, J., & Detha, A. I. R. (2022). Antimicrobial Properties of Lauric Acid and Monolaurin in Virgin Coconut Oil: A Review. ChemBioEng Reviews, 9(5), 442–461. https://doi.org/10.1002/cben.202100050

Omar Al, M., Wati, T., Wulandari, S. Y., Aryanti, U. F., Asmara, R. B., Ardiansyah, M. S., Amalulsyah, I., Lutfiah, M., Hijratullah, A., Istiqomah, N., & Syahrul. (2026). The Utilization of Lamtoro Plants as Animal Feed and Means of Reforestation in the Mecerit River, West Pengadangan Village, Pringgasela District, West Nusa Tenggara. Jurnal Pengabdian Masyarakat Tropis Indonesia, 2(1), 97–102. https://doi.org/10.65622/jpmti.v2i1.229

Pandiselvam, R., Jacob, A., & Manikantan, M. R. (2024). Coconut Based Food Products: Repertoire and Biochemical Features. In Coconut-Based Nutrition and Nutraceutical Perspectives (pp. 203–220). Springer Nature Singapore. https://doi.org/10.1007/978-981-97-3976-9_10

Ramesh, S. V., Pandiselvam, R., Thushara, R., Manikantan, M. R., Hebbar, K. B., Beegum, S., Mathew, A. C., Neenu, S., & Shil, S. (2020). Engineering intervention for production of virgin coconut oil by hot process and multivariate analysis of quality attributes of virgin coconut oil extracted by various methods. Journal of Food Process Engineering, 43(6). https://doi.org/10.1111/jfpe.13395

Salum, U., Foale, M., Biddle, J., Bazrafshan, A., & Adkins, S. (2020). Towards the Sustainability of the “Tree of Life”: An Introduction. In Coconut Biotechnology: Towards the Sustainability of the ‘Tree of Life’ (pp. 1–15). Springer International Publishing. https://doi.org/10.1007/978-3-030-44988-9_1

Srivastava, Y., Semwal, A. D., & Sharma, G. K. (2018). Virgin Coconut Oil as Functional Oil. In Therapeutic, Probiotic, and Unconventional Foods (pp. 291–301). Elsevier. https://doi.org/10.1016/B978-0-12-814625-5.00015-7

Strizhak, P. A., Volkov, R. S., Castanet, G., Lemoine, F., Rybdylova, O., & Sazhin, S. S. (2018). Heating and evaporation of suspended water droplets: Experimental studies and modelling. International Journal of Heat and Mass Transfer, 127, 92–106. https://doi.org/10.1016/j.ijheatmasstransfer.2018.06.10

Sun, G., Sun, S., & Ashfaq, T. (2025). Enzymatic Hydrolysis of Oilseeds and Their By‐Products for Controlled Aroma Formation: A Critical Review of Mechanisms and Applications. Comprehensive Reviews in Food Science and Food Safety, 24(5). https://doi.org/10.1111/1541-4337.70254

Thaweewong, P., & Anuntagool, J. (2023). Change in free cyanide content of bitter cassava during incubation and drying and physical properties of dry-milled cassava flour. Food and Bioproducts Processing, 138, 139–149. https://doi.org/10.1016/j.fbp.2023.01.009

Varghese, T., Monica, P., Sunil, C. K., & Natarajan, V. (2024). Processing and Preservation of Copra and Coconut Oil. In Preservation and Authentication of Coconut Products (pp. 105–141). Springer International Publishing. https://doi.org/10.1007/978-3-031-64653-9_6

Widianingrum, D. C., Noviandi, C. T., & Salasia, S. I. O. (2019). Antibacterial and immunomodulator activities of virgin coconut oil (VCO) against Staphylococcus aureus. Heliyon, 5(10), e02612. https://doi.org/10.1016/j.heliyon.2019.e02612

Xing, R., Wang, Y., Dai, J., Zhang, C., Hua, Y., Chen, Y., & Kong, X. (2025). Volatile flavor characterization of raw coconut milk and the impact of sterilization: A comparative analysis for coconut flavor preservation. Food Chemistry, 490, 145123. https://doi.org/10.1016/j.foodchem.2025.145123

Yao, M., Yang, Y., Fan, J., Ma, C., Liu, X., Wang, Y., Wang, B., Sun, Z., McClements, D. J., Zhang, J., Liu, L., Xia, G., Zhang, N., & Sun, Q. (2024). Production, purification, and functional properties of microbial fibrinolytic enzymes produced by microorganism obtained from soy-based fermented foods: developments and challenges. Critical Reviews in Food Science and Nutrition, 64(12), 3725–3750. https://doi.org/10.1080/10408398.2022.2134980

Yuniarti, I., Sapatra, E. F., Novia, S. F., Hovivah, H., Paramita, V., & Yulianto, M. E. (2021). Efficacy of a Natural Papain-Induced Enzyme on Virgin Coconut Oil-Based Hand and Body Lotions with Added Orange Peel Extract. International Journal of Technology, 12(3), 661. https://doi.org/10.14716/ijtech.v12i3.4070

Zhang, N., Li, Y., Wen, S., Sun, Y., Chen, J., Gao, Y., Sagymbek, A., & Yu, X. (2021). Analytical methods for determining the peroxide value of edible oils: A mini-review. Food Chemistry, 358, 129834. https://doi.org/10.1016/j.foodchem.2021.129834

Zhang, S., McClements, D. J., Zheng, R., Yu, X., Sun, Z., Xie, B., Chen, Y., & Deng, Q. (2025). A promising perspective to boost the utilizability of oil bodies: Moderate regulation and modification of interface. Comprehensive Reviews in Food Science and Food Safety, 24(3). https://doi.org/10.1111/1541-4337.70145

Author Biographies

Hana Safitri Putri Hadi, University of Mataram

Author Origin : Indonesia

Sarkono, University of Mataram

Author Origin : Indonesia

A.A. Ngurah Nara Kusuma, University of Mataram

Author Origin : Indonesia

Muh. Azhar Kholidi, National Sun Yat-sen University

Author Origin : Taiwan, Province of China

Downloads

Download data is not yet available.

How to Cite

Hadi, H. S. P., Sarkono, Kusuma, A. N. N., & Kholidi, M. A. (2026). Comparison of Antibacterial Activity and Physicochemical Characteristics of Vco (Virgin Coconut Oil) With Different Manufacturing Methods. Indonesian Journal of Tropical Biology, 2(1), 33–39. https://doi.org/10.65622/ijtb.v2i1.279